21 March 2013

New technique on laser dissection of neurons in PLOS ONE

P4L researchers from the Institut of Photonic Sciences (ICFO) led by Dr. Pablo Loza-Alvarez (SLN Facility head) presented in PLOS ONE Journal a new methodology for the assessment of the collateral damage induced by laser dissection of neurons in living soil worms. Their studies centered on the examination of the tissues surrounding the targeted neuron region using a combination of high resolution microscopy modalities.
Ultrashort pulsed lasers are the optimal tools for high precision surgery thanks to their enhanced tissue penetration, high peak powers and low pulse energy. In the case of the nematode Caenorhabditis elegans, these lasers have been proven to be particularly useful for successfully dissecting individual axons without affecting their viability. Of particular interest is the peculiar capability of the worm's neurons to regenerate axons after the laser dissection. Key to understanding the axon regeneration process is the ability to leave tissue components intact, assuring that any subsequently triggered mechanism would be related exclusively to the axon regeneration.
This work focused on the observation of any possible collateral damage attributed to the axon surgery and the minimization of said damage. ICFO researchers use a multimodal microscopy approach that is based on the simultaneous use of several advanced imaging techniques. This allows high resolution observation of many of the processes occurring around the targeted axon, providing a complete damage assessment tool that can be used during and after the neuron surgery. This work promises better understanding of the complicated process of axon regeneration in living organisms.

Link to article in PloS One.

Round Robin Experiment

Raman spectroscopy has already proved its effectiveness in many cases for medical diagnostics such as for cancer, cardiovascular diseases and infections. However, there are no standards in the different working groups, e.g. for sample preparation, implementation of the Raman experiments, spectra pre-treatment, data evaluation, etc.In a round robin experiment, the required groundwork will take place in order to define standardised Raman measurement methods, which will be fundamental for establishing Raman spectroscopy for clinical diagnostic procedures.

Latest News

5th International Conference on Biophotonics

20 March 2017

Continuing the success of the previous meetings held in Sacramento, Ottawa, Jena, and Florence, the 5th conference will come to Perth, Western Australia, running over two full days, and back-to-back with the Science on the Swan medical research conference, which delegates are strongly encouraged to also attend, at discounted rates. [more]

Biophotonics Wiki

Visit our wiki to consult our latest technologies and techniques. [more]


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